Advances in Urology

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  • Sperm were isolated, fixed in formaldehyde and processed with antibody KM without permeabilization as described for Sperm head assay, except that the fluorescently conjugated secondary antibodies were replaced by a 10 nm-gold-conjugated goat anti-mouse IgM Jackson Immunochemicals and the sperm were handled by centrifugation instead of being attached to microscopy coverslips.


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